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RNA structure is intimately related to function, yet methods to identify base-paired RNA strands in a transcriptome-wide manner in cells have remained elusive. One recent paper in Cell and two in Molecular Cell describe related methods to identify RNA sequences that interact in living cells, setting the stage for break-throughs in our understanding of RNA structure and function.
Just like proteins, RNA molecules adopt 3D structures that are important for their processing and function. In fact, nearly all textbooks depict messenger RNAs as linear, non-structured, single-stranded molecules. Not only do most RNAs likely have specific 3D structures, but these structures are almost certainly important for the functions of these RNAs.
Over the past several decades, a large toolbox of diverse methods has been developed to characterize RNA secondary structures and identify RNA sequences that base-pair with one another, whether in cis or in trans. These include computational modeling, phylogenetic analysis, genetic testing, and biochemical probing reviewed in Wan et al.
For example, several computational tools exist that use thermodynamic rules to predict RNA secondary structure based on the primary sequence. Comparative analysis of orthologous sequences from multiple species can also provide evidence supporting RNA secondary structures.
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